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. 2019 Jun 26;10:1456. doi: 10.3389/fimmu.2019.01456

Figure 2.

Figure 2

Antibody response in vaccinated mice after challenge infection. C57BL/6 female mice were immunized with subunit vaccine (pCDNA3.1 encoding TcG2 and TcG4) at day 0, boosted with subunit vaccine, fTr, or fTr+QA at day 21, and challenged with T. cruzi (10,000 parasites per mouse, intraperitoneal) at day 42. Mice were euthanized at day 63 (i.e., 21 days' post challenge infection). Sera samples were analyzed by an ELISA to evaluate the IgG (A–C), IgG1 (D–F), IgG2a (G–I), and IgG2c (J–L) levels captured with T. cruzi lysate (A,D,G,J) and recombinant TcG2 (B,E,H,K), and TcG4 (C,F,I,L) antigens. Sera samples from normal and infected mice were used as controls. Data (mean ± SD) are representative of duplicate observations per sample (n = 4 mice per group per experiment), and significance is annotated as * (none vs. vaccinated or infected vs. vaccinated/infected) and ∧ (comparison of vaccinated groups) (*,∧p < 0.05, **,∧∧p < 0.01, and ***p < 0.001).