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. 2019 Jun 26;10:1456. doi: 10.3389/fimmu.2019.01456

Figure 3.

Figure 3

Antigen presenting capacity of dendritic cells (DC) in vaccinated mice (± T. cruzi). Mice were vaccinated and challenged with T. cruzi trypomastigotes (10,000 parasites/mouse), as in Figures 1, 2. Mice were euthanized at 21 days' post-vaccination (pv) or 21 days' post-infection (pi). Single cell suspensions of spleen (A,C) and lymph nodes (B,D) of vaccinated (A,B) and vaccinated/infected (C,D) mice were labeled with fluorochrome-conjugated antibodies and analyzed by flow cytometry. Bar graphs show ex vivo percentage of Ly6GloCD11c+ splenic DC (SDC) and lymph node DC (LNDC) subsets that expressed markers of maturation (CD209+), stimulation (CD80+), antigen uptake (CD205+), and antigen presentation (MHCI+, MHCII+) in vaccinated and vaccinated/infected mice. SDC and LNDC from non-vaccinated/non-infected (none) and non-vaccinated/infected mice were used as controls. Data are presented as mean ± SD and representative of duplicate observations per sample (n = 4 mice per group per experiment). Significance is annotated as * (none vs. vaccinated or infected vs. vaccinated/infected) and ∧ (comparison of vaccinated groups) (*,∧p < 0.05, **,∧∧p < 0.01, and ***,∧∧∧p < 0.001).