Fig. 3.

Transcriptomic analyses of human adipocytes in relation to CD248 knockdown and hypoxia. Human adipocytes were differentiated in vitro and transfected at day 10 post-induction with siRNA oligonucleotides directed against CD248 (siCD248) or non-silencing control (Ctrl). After 48 h, cells were exposed to hypoxia (1% O₂) or continued normoxia (21% O₂) for 24 h. After this, cells were lysed and RNA subjected to gene microarray analyses. a. Principal component analysis demonstrating a clear separation between samples based on both CD248 knockdown and hypoxia. b. Venn diagram of the number of genes significantly (FDR 5%) down- (upper panel) or up-regulated (lower panel) by CD248 knockdown and hypoxia. c. Heat-map summarizing the log2-fold changes of the genes significantly (down- or up-) regulated by CD248-knockdown under hypoxic conditions. d. HIF-activity was determined by a luciferase-based hypoxia-response element (HRE) assay in human adipocytes treated as described above. *** = p < .001.