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. 2019 Jun 26;7:103. doi: 10.3389/fcell.2019.00103

FIGURE 1.

FIGURE 1

Fibroblasts lacking functional torsinA or LAP1 have increased deformability relative to controls. (A) Schematic illustration of PMF. Less deformable cells tend to occlude pores, thereby blocking fluid flow and resulting in an increased volume of fluid that is retained above the membrane. The retained fluid volume relative to the initial loaded volume is % retention. (B) PMF measurements of NIH3T3 fibroblasts expressing the indicated SS-EGFP-tagged torsinA constructs. (D,F) PMF measurements of the indicated MEF lines. PMF conditions: 10 μm pore size, 2.1 kPa for 50 s. Each data point represents the mean ± standard deviation (SD). Statistical significance was determined using Student’s t-test. (C,E,G) Cell size data. Boxplots show 25 and 75th percentiles; line shows median; and whiskers denote 10 and 90th percentiles. All data were obtained from three independent experiments. Statistical significance was determined using Mann-Whitney U-test. ∗∗∗p < 0.001; not significant (NS) p > 0.05.