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. 2019 Jun 26;9:225. doi: 10.3389/fcimb.2019.00225

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Copyright © 2019 Zhou, Li, Xu, Wang, Bai, Feng, Su, Chen, Wang, Liu, Wang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CEH combined with CDDP chemotherapy strategy can promote apoptosis. (A) Western blot analysis of the apoptosis-associated proteins: PARP, Casp8, Casp9, Casp3. (B) Western blot analysis of the apoptotic signal transduction pathway-associated proteins: TNFR, CYLD, JNK, p-JNK, Apaf-1, Bax, Bcl-2, and Cyto-c. β-Actin was used as the protein loading control. Immunohistochemical staining of c-PARP (C), c-Caspase 3 (D), and Bcl-2 (E). N, negative control; M, model control; CDDP, CDDP chemotherapy; CEH, cepharanthine hydrochloride chemotherapy; Comb, combined chemotherapy strategy.