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. 2019 Jun 26;9:225. doi: 10.3389/fcimb.2019.00225

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Copyright © 2019 Zhou, Li, Xu, Wang, Bai, Feng, Su, Chen, Wang, Liu, Wang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CEH enhances intestinal mucosal immunity. ELISA detected the peripheral blood immune factors, TNF-α (A), TGF-β (B), IL-2 (C), and IL-10 (D). (E) Western blot analysis of the TLR4 signaling pathway-associated proteins in small intestine tissue: NF-κB, TLR4, Myd88, JNK, and p-JNK. β-Actin was used as the protein loading control. MPO analysis was used to characterize neutrophil aggregation in the small intestine (F) and spleen (G). (H) HandE staining of paraffin sections for small intestine tissue. (I) Immunohistochemical staining of IgA. Error bars represent mean ± SD. N, negative control; M, model control; CDDP, CDDP chemotherapy; CEH, cepharanthine hydrochloride chemotherapy; Comb, combined chemotherapy strategy; ns, no significant difference. ^***P < 0.001.