Skip to main content
. 2019 Jun 26;10:663. doi: 10.3389/fphar.2019.00663

Figure 3.

Figure 3

Polydatin attenuated reactive gliosis and inflammatory mediators in MCAO injury. (A) Immunoreactivity of astrocytes (GFAP-positive cells) in the sham, MCAO, and Poly+MCAO groups are shown. Scale bar = 40 μm. The GFAP-positive cells were visualized by TRITC. Data presented are relative to Sham and number of experiment performed = 3, and (n = 5–7/group). (B) Effect of polydatin on inflammatory cytokines. Western blot analysis of TLR4, TNFα, and IL-1β (n = 5–8/group). The Western blots were quantified using ImageJ and analyzed by GraphPad Prism 5 software. β-Actin was used as a control. (C) Immunohistochemistry results for IL-1β are shown; scale bar = 50 µm. (D) Double immunofluorescence reactivity of TNF and Iba-1. Scale bar = 20 μm. The cortical sections show correspondingly elevated expression of TNF and Iba-1 after 24 h of permanent ischemia. TNF is visualized with FITC and IBa-1 by TRITC (n = 5-7/group). ***p < 0.001; **, ## p < 0.01; # p < 0.05.