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. 2019 Jun 26;10:663. doi: 10.3389/fphar.2019.00663

Figure 4.

Figure 4

Effect of polydatin on oxidative stress. (A) Western blot analysis of COX2, iNOS, and p-NF-κB (n = 5–8/group). The protein bands were quantified using ImageJ and analyzed with GraphPad Prism 5 software. β-Actin was used as a control. Densitometric analysis was expressed in arbitrary units. (B) Representative photos of immunohistochemistry for p-NF-κB and iNOS; scale bar = 50 µm. (C) Double immunofluorescence reactivity of p-NF-κB and TRX. Scale bar = 20 μm and (n = 5–7/group). The ischemic tissue showed elevated expression of p-NF-κB and attenuated expression of TRX after 24 h of continuous ischemia. p-NF-κB and TRX were visualized by FITC and TRITC, respectively. (D) Representative immunofluorescence images of 8-oxoguanine staining (n = 5–7/group). Scale bar = 50 μm. (E) Polydatin treatment increased the levels of several antioxidant proteins in ischemic cortex. Western blot analysis of Nrf2, HO-1, and TRX (n = 5–8/group). The protein bands were quantified using ImageJ and analyzed by GraphPad Prism 5 software. β-Actin was used as a control. ***p < 0.001; ##, **p < 0.01; #, *p < 0.05.