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Oligomer formation by PFO and PFO variants. Oligomer formation was assessed by incubating PFO and its derivatives with POPC-cholesterol liposomes for 30 min at 37°C. After incubation, sample buffer was added to the proteoliposomes with or without heating them to 95°C for 3 min. Oligomers were resolved on a 1.5% SDS agarose gel. M, PFO monomer without liposomes and heat.
