Fig. 5.

Rb1 increases macrophage phagocytosis in the mouse lung. (A–C). Mice were injected intraperitoneally with Rb1 (0 or 5 mg/kg) for 2 h and bronchoalveolar lavage (BAL) cells were obtained from mouse lungs. The cell lysates were subjected to electrophoresis in polyacrylamide gels. (A) Representative western blots and (B and C) quantitative density ratio of phospho-p38 or total-p38 and Akt from five mice in each group. *p < 0.05 compared to the control. (D–E) Mice were injected intraperitoneally with Rb1 (0 or 5 mg/kg) for 2 h and FITC-conjugated E. coli was added to mouse lungs for 2 h. BAL cells isolated from the lungs were assayed by flow cytometry. (D) Representative flow cytometry histograms are shown. (E) Each bar represents the mean ± SD (n = 4). *p < 0.05 compared to the control. FITC, fluorescein isothiocyanate; SD, standard deviation.