Fig. 1.

High expression levels of PARP-1 and PARG in the iPS cells. (a) PARP-1 and PARG were analyzed via Western blotting. GAPDH was used as a loading control. (b) PARP activity assay. All the samples in the 96-well plates were read on a Wallac ARVO SX 1420 Multilabel Counter (PerkinElmer) using the wavelength settings for optimized fluorescence. The vertical axis represents RFU (relative fluorescence units). The experiments were performed on four biological replicates, for each cell line. Comparison of the two groups (progenitor cells and reprogrammed iPS cells) with normally distributed variables was performed using a Student's t test analyzed by a Caleida graph. Statistical significance was defined as a value of p < 0.05. Data are expressed as the mean ± SEM.