Figure 1.

Overall Workflow of LIGS

Step one: SELEX was performed against Jurkat.E6 cells up to the 11^th round. At the 12^th round, a negative SELEX step was introduced, using BJAB cells to remove nonspecific DNA sequences. Step two: the enriched cell-SELEX library against Jurkat.E6 cells was divided into two fractions. The first fraction was utilized in LIGS, using multiple mAbs and Jurkat.E6 cells. The second fraction was used for an additional SELEX cycle, utilizing primary T cells isolated from peripheral blood mononuclear cells (PBMCs). The resulting library from this step was then used in LIGS with multiple mAbs and primary T cells. Step three: the resulting eluted sequences from each mAb were subjected to Illumina high-throughput sequencing (HTS), followed by bioinformatics analysis. Step four: specific aptamer sequence hits against TCR-CD3 expressed on T cells were identified and validated.