Figure 5.

Characterization of Aptamers for Cell Specificity

(A) The overall conclusion from three independent flow-cytometric analyses of ZUCH-1, ZOKT-2, ZUCH-3, ZUCH-4, and ZUCH-5 for cell specificity using Jurkat.E6 and three negative control cells: BJAB, MOLT-3, and Toledo. Percent fluorescent intensity values on the y axis were determined by normalizing against a random control sequence using the equation ($\left(aptamer-random/random\right)\times 100$ (one-way ANOVA using Tukey’s multiple comparisons test performed on GraphPad Prism to obtain statistical significance: ***p = 0.0001; ****p < 0.0001). See Figure S6 for corresponding histograms. (B) Cross-competition experiments among ZOKT-2, ZUCH-3, ZUCH-4, and ZUCH-5, using unlabeled ZUCH-1 as a competitor against Jurkat.E6 cells, demonstrating that all five aptamers compete toward the same target on Jurkat cells. (C) The overall conclusion from three independent analyses of cross-competition experiments with unlabeled ZUCH-1 against ZOKT-1, ZUCH-3, ZUCH-4, and ZUCH-5 (two-way ANOVA and the Holm-Sidak multiple comparisons test performed on GraphPad Prism to obtain statistical significance: *0.01 ≤ p ≤ 0.05; **0.001 ≤ p ≤ 0.01; ***0.0001 ≤ p ≤ 0.001).