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. 2019 Apr 1;28(14):2339–2351. doi: 10.1093/hmg/ddz059

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© The Author(s) 2019. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Salbutamol treatment alters MuSK immunoreactivity in ColQ^−/− mice. A. AChR and MuSK clusters labelled with Alexa 488 α-BTX (green, AChR) and anti-MuSK antibody (red) respectively on transverse sections of gastrocnemius muscle from 10-wk-old WT, water treated ColQ^−/− (ColQ^−/− WFI) and salbutamol treated ColQ^−/− (ColQ^−/− SAL) mice. Scale bar, 20 μm. B. Quantification of MuSK fluorescence intensities from projection of confocal stacks. MuSK fluorescence intensity per AChR cluster was decreased in ColQ^−/− muscle, and significantly increased in salbutamol treated ColQ^−/− mice compared to water treated. n = 180 NMJs per group for B. Unpaired Student’s t tests. ^∗∗∗p < 0.001, ^∗p < 0.05, n.s. not significant.