Figure 2.

Distribution of the major GH families across rumen microbial genomes. Phylogeny of the 501 16S RNA sequences from the Hungate1000 collection (Seshadri et al. 2018). The sequences were aligned, and the tree was constructed using MEGA7.0 software. The tree displayed in the figure was condensed by collapsing branches with less than 50% statistical significance. Phyla and selected classes are indicated using either a color range that covers the full clades or branch symbols. Major cellulase GH families 5, 6, 8, 9, 45 and 48 are indicated with a green color gradient, major hemicellulase GH families 10, 11, 26, 30, 43 and 74 with a blue gradient, and GH13 enzymes (amylases) are indicated in yellow. The multiple bars indicate both the presence and quantity of each GH within each microbial genome.