FIG 2.

Localization of the first replisome in relation to the pilus pole of B. bacteriovorus. B. bacteriovorus cells (red) with replisome (green) localized in the vicinity of a pole (flagellar or pilus) or in the midcell are shown. The white arrow indicates the pilus pole, determined by watching predator entry, which is pilus first. A schematic of a B. bacteriovorus cell is depicted on the right. The cell is divided into subregions according to the percentage of cell length. Images were recorded every 60 s. Red indicates PilZ-mCherry-labeled cytoplasm of attack-phase B. bacteriovorus cells, and green indicates DnaN-mNeonGreen of B. bacteriovorus. All photos represent merged bright-field and fluorescence (red and green) images. The B. bacteriovorus and E. coli cells are marked by yellow and white dotted lines, respectively. Bar, 1 μm.