FIG 2.

Phylogenetic and structural identification of WYHR-1 cells using the coupled FISH-SEM approach. (a to c) Fluorescence microscope images of WYHR-1 cells hybridized by the 5′-FAM-labeled universal bacterial probe EUB338 (green) and the 5′-Cy3-labeled WYHR656 probe (red). All bacteria were labeled fluorescently with the EUB338 probe (green in panel a). Only three bacteria were labeled fluorescently with the WYHR656 probe (red in panel b), overlapping (yellow-red in panel c). (d) Coupled SEM image of the same microscopic field as in panel c. (e to g) High-magnification SEM image of WYHR-1 cells labeled 1 to 3 in panel d and marked by red arrows in panels c and d. (h to j) High-magnification SEM image of other MTB cells labeled 4 to 6 in panel d and marked by blue arrows in panels c and d.