TABLE 2.
Onboard sequencing pipeline results for sequencing performancea
| Chip type (Ion v2) | No. of runs | Setting | Total no. of bases (Mbp) | Total no. of reads (Q20) | Loading (%) | Mean length (bp) | Median length (bp) |
|---|---|---|---|---|---|---|---|
| Ion 314 | 5 | Default | 83 | 3.06 × 105 | 72 | 265 | 258 |
| Stringent | 56 | 1.48 × 105 | 379 | 439 | |||
| Difference | −33% | −52% | 114 | 181 | |||
| Ion 316 | 1 | Default | 465 | 1.49 × 106 | 52 | 312 | 364 |
| Stringent | 322 | 8.47 × 105 | 380 | 433 | |||
| Difference | −31% | −43% | 68 | 69 | |||
| Ion 318 | 2 | Default | 551 | 2.12 × 106 | 50 | 259 | 248 |
| Stringent | 404 | 1.17 × 106 | 345 | 362 | |||
| Difference | −27% | −45% | 86 | 114 | |||
a
Torrent Suite analysis of raw sequencing data was performed using three different sequencing chip types (Ion v2 314, 316, and 318) and two analysis methods (default and stringent). Loading refers to the percentage of wells of each sequencing chip that are filled with ISPs.