Concentrations of sewage-associated marker genes in nonhuman fecal samples. (A) Gene copies (GC) of HF183, Lachno3, BacV6-21, and crAssphage CPQ_056 markers in animal hosts. Results are calculated in GC/ng of DNA and GC/g of feces. Each animal host is represented in unique color and shape. Error bars represent marker GC means ± standard deviations. (B) Overall nonhuman sample test results for six markers where the bar height represents sample numbers. Quantifiable sample numbers are shown in maroon bars, negative sample numbers are shown in light blue bars, and detectable but not quantifiable (positive) numbers are shown in gray bars.