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. 2019 Jul 1;85(14):e00622-19. doi: 10.1128/AEM.00622-19

FIG 4.

FIG 4

C. acetobutylicum thlsupFAST shows improved fluorescence over time compared to C. acetobutylicum thlFAST, but C. acetobutylicum adcFAST, and C. acetobutylicum ptbFAST show higher fluorescence intensity than C. acetobutylicum adcmodFAST and C. acetobutylicum ptbmodFAST over time as determined with a fluorescence microplate reader. (A) C. acetobutylicum thlFAST fluorescence intensity and growth curve over ∼35 h in Turbo CGM plus erythromycin using a fluorescence microplate reader (n = 2). (B) C. acetobutylicum thlsupFAST fluorescence intensity and growth curve in Turbo CGM plus erythromycin or Turbo CGM over ∼36 h using a fluorescence microplate reader (n = 3). (C) C. acetobutylicum adcFAST fluorescence intensity and growth curve over ∼36 h in Turbo CGM plus erythromycin using a fluorescence microplate reader (n = 2). (D) C. acetobutylicum adcmodFAST fluorescence intensity and growth curve over ∼36 h in Turbo CGM plus erythromycin using a fluorescence microplate reader (n = 2). (E) C. acetobutylicum ptbFAST fluorescence intensity and growth curve over ∼37 h in Turbo CGM plus erythromycin using a fluorescence microplate reader (n = 2). (F) C. acetobutylicum ptbmodFAST fluorescence intensity and growth curve over ∼36 h in Turbo CGM plus erythromycin using a fluorescence microplate reader (n = 2). Error bars indicate SD. Lag times were standardized between fermentations by normalizing an OD600 of 1.0 at hour 10 of growth as previously described (49).