FIG 5.

Sorting low (25%)- and high (25%)-fluorescence populations of C. acetobutylicum thl^supFAST shows that FAST is expressed in both vegetative and clostridial morphologies and low-fluorescence cells remain in a vegetative state. (A) C. acetobutylicum thl^supFAST histogram (top) of low fluorescence (P1) and high fluorescence (P2) and dot plots (bottom) with accompanying phase-contrast images of sorted cell populations after 32 h of growth. (B) C. acetobutylicum thl^supFAST histogram (top) of low fluorescence (P1) and high fluorescence (P2) and dot plots (bottom) with accompanying phase-contrast images of sorted cell populations after 56 h of growth. Scale bars in insets represent 5 μm.