TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| NEB 5-alpha | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB |
| ER2275 | hsdR mcrA recA1 endA1 | NEB |
| C. acetobutylicum | ||
| ATCC 824 | Wild-type strain | ATCC |
| Plasmids | ||
| pAN3 | Kmr; Φ3T I gene | 46 |
| pSOS94_MCS | ptb promoter; Ampr MLSr ColE1 Ori repL MCS | 46 |
| pSOS95_MCS | thl promoter; Ampr MLSr ColE1 Ori repL MCS | 43 |
| p95thlFAST | thl promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95thlsupFAST | thlsup promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95adcFAST | adc promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95adcmodFAST | adcmod promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95ptbFAST | ptb promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95ptbmodFAST | ptbmod promoter; Ampr MLSr ColE1 Ori repL FAST | This study |
| p95ZapA-FAST | thl promoter; Ampr MLSr ColE1 Ori repL ZapA FAST | This study |