FIG 4.
P350-dependent production of bovine EKL by K. lactis YCT1267 cultured in shake flasks. The cultures were grown in YDFM containing 1% glucose as the sole carbon source. Shown are (A) glucose consumption, (B) EKL expression, (C) oxygen consumption, and (D) ethanol production relative to hours of culturing. In panels A and D, ND represents the glucose (<0.002%) and ethanol (<0.05%) detection limits of our instrumentation. Error bars (and light gray shading in panel B) represent the standard deviation from the mean of three independent cultures.