FIG 3.
Determination of the relative transcriptional levels of lutP gene in BC medium using l-lactate as the stimulator in B. coagulans DSM1 ΔldhL1 ΔldhL2 (A) and glucose as the inhibitor in B. coagulans DSM1 (B). The threshold cycles (CT) for each PCR with different concentrations of cDNA were determined and were compared with that for a standard DNA (the 16S rRNA gene) that was also analyzed at the same time. The y axis indicates the transcription ratio of lutP at 5 min, 10 min, and 30 min to 0 min. Error bars represent SDs calculated from three independent determinations.