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. 2019 Jul 1;85(14):e00672-19. doi: 10.1128/AEM.00672-19

FIG 5.

FIG 5

Identification of the binding sites. (A) Nucleotide sequences of the lutP-lutR intergenic region of B. coagulans DSM1. F0 (218 bp) is the lutP-lutR intergenic region, F1 (73 bp) is the fragment that does not contain an inverted repeat sequence. F2 (75 bp) is the fragment with the inverted repeat sequence of TCATCTGATGA. F3 (70 bp) is the fragment with two inverted repeat sequences of AATTGCAATT and TTTTTCAAAAA. F2− is the fragment with the inverted repeat sequence of F2 deleted. (B) BLI binding assay of LutR to fragment F1. (C) BLI binding assay of LutR to fragment F2. (D) BLI binding assay of LutR to fragment F3. (E) BLI binding assay of LutR to fragment F2−. Different concentrations of 0.15625, 0.3125, 0.625, 1.25, and 2.5 μM purified LutR injected over a Sensor Chip with biotinylated intergenic DNA regions. The vertical dashed lines denote phase I, association with biotinylated intergenic DNA regions, and phase II, dissociation in binding buffer.