FIG 6.

Effects of l-lactate on the DNA-binding activity of LutR. The purified LutR protein was incubated with 5 mM l-lactate in sodium phosphate buffer (20 mM, pH 8.0) for 20 min before BLI binding assay of fragment F2. Different concentrations of 0.15625, 0.3125, 0.625, 1.25, and 2.5 μM purified LutR injected over a Sensor Chip with biotinylated intergenic fragment F2. The vertical dashed line denotes phase I, association with biotinylated fragment F2, and phase II, dissociation in binding buffer.