TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pHT315-gfp | Contains the gfp-mut1 coding sequence between the XbaI and HindIII restriction sites of plasmid pHT315 | 66 |
| pHT304-18ΩmCherry-gfp | Contains a ribosome binding site and mCherry (optimized for Bacillus spp.) and gfp coding sequences | Gift from M. Gohar and L. Slamti |
| pHT315-PcshA′gfp | Fluorescent reporter plasmid construct for cshA transcriptional activity | This study |
| pHT304-18ΩPabrB′mCherry-gfp | Fluorescent reporter plasmid construct for abrB transcriptional activity | This study |
| B. cereus strains | ||
| ATCC 14579 | Wild type | 67 |
| ATCC 10876 | Wild type | 68 |
| MM3 | Wild type | 69 |
| ATCC 14579-PcshA′gfp | ATCC 14579 carrying pHT315-PcshA′gfp | This study |
| ATCC 10876-PcshA′gfp | ATCC 10876 carrying pHT315-PcshA′gfp | This study |
| MM3-PcshA′gfp | MM3 carrying pHT315-PcshA′gfp | This study |
| ATCC 14579-PabrB′mCherry | ATCC 14579 carrying pHT304-18ΩPabrB′mCherry | This study |
| ATCC 10876-PabrB′mCherry | ATCC 10876 carrying pHT304-18ΩPabrB′mCherry | This study |
| MM3-PabrB′mCherry | MM3 carrying pHT304-18ΩPabrB′mCherry | This study |