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Primer sequencesa
| Primer | Sequence | Tm (°C) |
|---|---|---|
| PcshA-Fw-EcoRI | GCAGGAATTCCAAATTGCTGAAGGAGCAAA | 55.2 |
| PcshA-Rv-BamHI | TGCTGGATCCAAAAGGCGTTTTCCGAATTT | 55.2 |
| PabrB-Fw-SphI | GCGCATGCTCGTATAAGCATGTCCAATG | 56.3 |
| PabrB-Rv-XbaI | CGCGTCTAGAAGATTTCAAGAGCGTCCTTT | 56.5 |
Restriction sites are underlined. Tm, melting temperature.
