Fig. 1.

HNF1A expression is significantly associated with gemcitabine sensitivity in PDAC cell lines. (A) HNF1A expression levels were detected in seven pancreatic cancer cell lines by qRT-PCR. Data are shown as fold change (2^−∆∆CT) and the mean ± SD from three independent experiments. (B–C) HNF1A expression levels were evaluated by qRT-PCR in PANC-1 and MIA PaCa-2 cell lines after different dose (0, 1, and 10 μm) of gemcitabine treatments for 12 h. (D–E) The protein levels of HNF1A were evaluated by Western Blot in PANC-1 and MIA PaCa-2 cell lines after different dose (0, 1, and 10 μm) of gemcitabine treatments for 12 h. (F) Immunofluorescence staining showed an inhibition of HNF1A expression in nucleus after gemcitabine treatment. The images shown are representative results of three independently repeated experiments and are presented as mean ± SD. Scale bar, 50 μm. *p < .05, **p < .01 (Student's t-test).