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. 2019 Jun 4;17:185–197. doi: 10.1016/j.omtn.2019.05.018

Figure 3.

Figure 3

miR-221 Inhibits myoFB Activation and Collagen Synthesis

Adult rat cFB was serum starved for 24 h, then transfected with miR-221 mimic or control (miR-221 and MC) and incubated for a specified period of time. (A) qRT-PCR and (B) western blot measurements of α-SMA mRNA and protein expressions. (C) Collagen gel contraction was measured as the percentage reduction of gel area over the culture surface after 48-h pre-stress and 48-h free contraction. (D) qRT-PCR measurement of mRNA expression of collagen I (Col1a1 and Col1a2), collagen III (Col3a), fibronectin (Fn1), and its extra domain A isoform (Fn-EDA). (E) Sircol collagen assay of cFB supernatants 7 days after mimic transfection. (F) qRT-PCR and (G) western blot measurement of Smad2 and Smad3 mRNA and protein expressions. Antibodies recognized phosphorylated (P-SMAD2/3, Serine 423/425) and total (T-SMAD2/3), respectively. *p < 0.05, **p < 0.01, ***p < 0.001 versus MC. n = 3 in triplicates.