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. 2019 Jun 18;17:74–86. doi: 10.1016/j.isci.2019.06.019

Figure 3.

Figure 3

Caspase Inhibitors Block DNA Damage-Induced Cell Death in RARγ-KO Cells

(A) Rarγ+/+ and Rarγ−/− cells were pre-treated for 30 min with or without z-VAD-fmk (zVAD), zLEHD, or combined treatments as indicated followed by treatment with cisplatin 50 μM (left panel) or etoposide 50 μM (right panel) for 36 h. Cell survival was determined by propidium iodide (PI) staining and analyzed by flow cytometry.

(B) Rarγ+/+ and Rarγ−/− cells were pre-treated for 30 min with or without zVAD, zLEHD, NEC1, or combined treatments as indicated followed by treatment with cisplatin 50 μM (left panel) or etoposide 50 μM (right panel) for 36 h. Cell survival was determined by PI staining and analyzed by flow cytometry.

(C) Rarγ+/+ and Rarγ−/− cells were pre-treated for 30 min with or without combined z-VAD-fmk (zVAD) and zLEHD followed by treatment with cisplatin (left panel) or etoposide (right panel) for 20 h. Cell lysates were analyzed by immunoblotting as indicated. All blots above are representative of one of three experiments. See also Figure S4. Results shown are averages ± SEM from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.