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. 2019 Jun 18;17:74–86. doi: 10.1016/j.isci.2019.06.019

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Cytosolic RARγ Is Required for RIPK1 to Initiate Necroptosis in Response to DNA Damage

(A) Rarγ^+/+ cells were treated with cisplatin for the indicated time period. Cell lysates were collected and immunoprecipitated with anti-RARγ antibody. The immunoprecipitated complexes were immunoblotted with the indicated antibodies.

(B) Rarγ^+/+ and Rarγ^−/− cells were treated with cisplatin for the indicated time period. Cell lysates were collected and immunoprecipitated with anti-caspase 8 antibody. The immunoprecipitated complexes were immunoblotted with the indicated antibodies. See also Figure S5.

(C) Confocal microscopy of WT MEFs transfected with GFP- RARγ plasmid and treated with cisplatin, and images were captured in the indicated time period (blue, DAPI; green, RARγ). Images are representative of one of three experiments. Scale bar, 10 μm.

(D) Cellular fractionation of Rarγ^+/+ cells treated with DMSO or cisplatin 50 μM for 6 h was performed. Total (T), cytosolic (C), and nuclear (N) fractions were analyzed by immunoblotting with the indicated antibodies. All blots and images above are representative of one of three experiments.