Figure 1.

Intracellular Innate Immune Responses Are Activated by AAV Gene Therapy

AAV2.GFP vector (1 × 10⁹ gc) was subretinally injected into wild-type C57BL/6 mice, with sham injections of PBS undertaken in the contralateral eye. RNA was extracted from the mouse retina on days 3, 7, and 15 post-injection (n = 3/time point). The expressions of a range of cytosolic anti-viral (A) sensor and (B) effector genes and (C) two genes from the APOBEC family were quantified using qRT-PCR. Relative expression was calculated as a mean fold change (±SEM) relative to the mean of both eyes from uninjected baseline controls (n = 2). *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001 (two-way ANOVA with Šídák’s multiple comparison test).