Fig. 5.

CNO administration to hM3Dq-expressing ARN GABA neuron fibers in the rPOA elicits LH secretion in male and female mice. (A) Schematic of the regions targeted in a sagittal mouse brain map for transfection and CNO delivery. (B,C) Representative confocal images showing ARN GABA projecting fibers transfected with AAV5-hM3Dq-mCherry (red) nearby GnRH neurons (blue) in the rPOA at low and high magnification. Scale bar in B = 100 μm and in C = 5 μm. (D) Protocol timeline. (E,F) Representative LH secretion pattern from ARN GABA^{OFF Target} male and female controls in response to CNO delivery and peripheral GnRH administration. (G,H) Representative examples of circulating LH secretion patterns in response to CNO delivery into the rPOA of correctly targeted ARN GABA^hM3Dq+/rPOA^ON male (blue traces) and female (red traces) mice. (I-J) Mean ± SEM LH responses in ARN GABA^hM3Dq+/rPOA^ON males (blue) and females (red) and in corresponding control animals (grey). Purple triangles indicate LH levels significantly higher (p < .05) than baseline levels. Two-way ANOVA with Dunnett's post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)