Fig. 7.

The effect of chronic ARN GABA neuron activation on testosterone and LH pulse frequency in female mice. (A,B) Individual levels of circulating testosterone levels before (black dots) and during treatment with CNO in the drinking water (orange squares) in ARN GABA ^hM3Dq+ (N = 13) and controls (consisting of ARN GABA^{OFF Target} (N = 5) and ARN GABA^mCherry (N = 6) mice. (C) Percentage change in testosterone in response to CNO compared between control and ARN GABA^hM3Dq+ mice. (D-F) Representative examples of LH release profiles over 2 h in individual ARN GABA ^hM3Dq+ and ARN GABA^{OFF Target} animals at the end of 2 weeks of water only (black line and dots) and then following 2 weeks of CNO administration through the drinking water (orange line and square). (G,H) LH pulse frequency before and after CNO treatment period, and (I,J) cumulative LH secretion (area under the curve) before and after CNO treatment period in ARN GABA ^hM3Dq+ (N = 11) and ARN GABA^{OFF Target} (N = 5) groups. (A,B,G-J) Paired Student's t-tests; (C) Mann-Whitney U test; *** p < .001.