Figure 3.

Heparan Sulfate Accumulation in Cultured Primary Astrocytes, Neurons, and Microglia Derived from NAGLU-Null Mouse E18 Embryos

Primary cultures of neurons, astrocytes, and microglia were established from brain tissues taken from wild-type and NAGLU-null mouse embryos (E18) and cultured to allow for accumulation of HS in affected cells. HS was prepared, and total HS was measured by quantitative HS analysis. Results are shown as mean picomoles total HS/microgram protein ± SD in triplicate wells.