Figure 4.

Effects of NAGLU-IGF2 and Unmodified NAGLU on MPS IIIB Primary Mouse Neurons

(A) HS levels in three replicate wells in a 96-well plate after transient 30 min exposure of MPS IIIB neurons to different concentrations of either NAGLU-IGF2 (blue bars) or unmodified NAGLU (gray bars) were measured 48 h after treatment and compared to levels in replicate vehicle-treated MPS IIIB neurons (white bar) or wild-type neurons (black bar). The results (the mean ± SD) are shown as picomoles total HS/microgram protein. (B) Cellular uptake of exogenous NAGLU-IGF2 (blue trace) or unmodified NAGLU (gray trace) after 30 min exposure by primary embryonic cortical neurons. (C) Immunofluorescence was measured after cortical neurons were exposed for 30 min to either NAGLU-IGF2 or unmodified NAGLU. Permeabilized cells were immunostained with an antibody to NAGLU (red; 1:800) and an antibody to the neuronal marker MAP2 (green; 1:500). Scale bar, 10 μm.