Figure 5.

Effects of NAGLU-IGF2 and Unmodified NAGLU on MPS IIIB Primary Mouse Astrocytes

(A) HS levels in three replicate wells in a 96-well plate after a transient 30 min exposure of MPS IIIB astrocytes to different concentrations of either NAGLU-IGF2 (blue bars) or unmodified NAGLU (gray bars) were measured 48 h after treatment and compared to levels in replicate vehicle-treated MPS IIIB astrocytes (white bar) or wild-type astrocytes (black bar). The results (the mean ± SD) are shown as picomoles total HS/microgram protein. (B) Cellular uptake of exogenous NAGLU-IGF2 (blue trace) or unmodified NAGLU (gray trace) by primary embryonic astrocytes after a 30 min exposure. (C) Immunofluorescence was measured in permeabilized astrocyte cultures previously exposed for 30 min with either NAGLU-IGF2 or unmodified NAGLU. NAGLU antibody (red; 1:800), astrocyte marker GFAP (green; 1:500), and Hoechst dye for nuclei (blue; 1 μg/mL). Scale bar, 10 μm.