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. 2019 May 28;14:47–55. doi: 10.1016/j.omtm.2019.05.010

Figure 1.

Figure 1

Susceptibility of iPSCs to REVec and Its Effect on Cell Viability

(A) Human iPSCs (110, 112, 116, BMI, Epi, 201B7, and 409B2) were transduced with replication-defective REVec at a MOI of 1.0. At 1 week post-transduction, expression of GFP and pluripotency marker SSEA4 was analyzed (top panels). The percentage of GFP-positive cells was determined and reported as compared to mock (bottom panels). Scale bars, 100 μm. (B) Cell viability was determined by WST-1 assay at 1 week post-transduction and presented as percentage compared to mock. Data are shown as averages of two independent experiments with error bars represending SEM.