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. 2019 Apr 17;20(7):e46436. doi: 10.15252/embr.201846436

Figure EV2. CDK2 genetic depletion increased endogenous levels of NICD in HEK293T cells.

Figure EV2

  1. CDK2−/− HEK293T cells, lacking endogenous CDK2, were cultured for 48 h after seeding followed by Western blot for NICD, CDK2, CDK1 and CDK8. β‐Actin served as loading control.
  2. Quantification of the density of Western blot bands in (A) using ImageJ software. Data are expressed as fold changes compared to WT cells. All data represent the mean ± SEM from three independent experiments. Student's t‐test analysis was performed, with *P ≤ 0.05.
  3. CDK2−/− HEK293T cells were cultured for 48 h after transfection with plasmids encoding scrambled siRNA (−) or siRNA specific for CDK1 (+) followed by Western blot for NICD, CDK2, CDK1 and CDK8. β‐Actin served as loading control.
  4. Quantification of the density of Western blot bands in (C) using ImageJ software. Data are expressed as fold changes comparing WT cells (−/+ CDK1 siRNA) and CDK2−/− cells (−/+ CDK1 siRNA). All data represent the mean ± SEM from three independent experiments. Student's t‐test analysis was performed, with *P ≤ 0.05 and ***P ≤ 0.001.