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. 2019 May 22;20(7):e47563. doi: 10.15252/embr.201847563

Figure EV4. USP7 is involved in the p53‐regulated H2Bub1 levels.

Figure EV4

  1. H1299 cells were transfected with an empty vector (Flag) or a Flag‐tagged p53 together with a control siRNA (siCont.) or two separate USP7‐specific shRNAs (siUSP7) for 48 h. Western blot analysis was then performed.
  2. Western blot analysis of 293T cells transfected with a control siRNA or two separate USP7‐specific shRNAs (siUSP7).
  3. Results of the NLS prediction analysis.
  4. Relative quantification of 1N–3N bands specifically corresponding to the data in Fig 4G. No replicates were involved in the band quantification.