Knockdown of LIMD2 inhibits the malignant phenotypes of non-small cell lung cancer cells. H1975 and A549 cells were transfected with siLIMD2 or siNC. Following transfection, (A) reverse transcription-quantitative PCR and (B) western blotting were used to examine the mRNA and protein expression levels of LIMD2. Cell Counting Kit-8 for (C) H1975 and (D) A549, wound healing for (E) H1975 and (F) A549 and Transwell assays for (G) H1975 and (H) A549 cells were used to examine cell proliferation, migration and invasion, respectively. Magnification for wound healing assay was ×40. Magnification for Transwell assays was ×400. **P<0.01 vs. siNC. LIMD2, LIM domain containing 2; si, small interfering RNA; NC, negative control; OD, optical density.