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. 2019 Jun 19;18(2):1607–1616. doi: 10.3892/ol.2019.10496

Figure 2.

Figure 2.

Upregulation of miR-362-5p reverses the cisplatin resistance of SGC7901/DDP cells. (A) Light microscopy (magnification, ×100) and (B) lentiviral transfection assay of SGC7901/DDP cells (magnification, ×100). Cells that were successfully transfected expressed green fluorescent protein. (C) Reverse transcription-quantitative PCR analysis demonstrated that the expression level of miR-362-5p was markedly increased in SGC7901/DDP cells transfected with miR-362-5p mimic compared with NC-transfected and untransfected cells. (D) Viability of cells treated with cisplatin for 48 h. MTT assays were conducted on SGC7901/DDP cells treated with various concentrations of cisplatin (0, 0.75, 1.25, 2.5, 5, 10 or 20 µg/ml). The IC50 for cisplatin was estimated based on the cell viability. The IC50 for cisplatin of the miR-362-5p mimic-transfected cells was notably lower compared with the controls. n=3; ***P<0.001 vs. SGC7901/DDP-NC and SGC7901/DDP; **P<0.01 vs. SGC7901/DDP-NC and SGC7901/DDP; IC50, median inhibitory concentration; miR-362-5p, microRNA-362-5p; NC, negative control.