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. 2019 Jun 14;18(2):1922–1930. doi: 10.3892/ol.2019.10480

Figure 4.

Figure 4.

Identification of CCL8 as a direct target of miR-181. (A) Predicted binding sites of miR-181 in the wild-type and mutant 3′-UTR of CCL8. (B) Luciferase activity assay of cells containing wide-type or mutant CCL8-3′-UTR following transfection with miR-181 or miR-NC. (C) Protein expression of CCL8 in U87 and A172 cells transfected with miR-181 or miR-NC. (D) mRNA expression of CCL8 in U87 and A172 cells transfected with miR-181 or miR-NC. (E) Protein expression of CCL8 in U87 and A172 cells transfected with pcDNA-CCL8 and pcDNA. All data are shown as the mean ± standard deviation and based on at least three independent experiments. *P<0.05. miR-181, microRNA-181; UTR, untranslated region; CCL8, C-C motif chemokine ligand 8; miR-NC, miR-negative control.