Fig. 2.

Scheme of generating a ‘Cas9’ strain for gene editing. A fungus is transformed through protoplasts or Agrobacterium tumefaciens-mediated transformation with the CAS9 gene under control of either an inducible or constitutive promoter generating a ‘Cas9’ strain. Once the CAS9 gene has been stably integrated into the fungal genome, subsequent transformations allow various other loci of interest to be targeted by sgRNAs.