Skip to main content
. 2019 May 27;44:150–161. doi: 10.1016/j.ebiom.2019.05.046

Fig. 1.

Fig. 1

FOX-A1 is upregulated in docetaxel-resistant LAD cells and insensitive LAD tissues and correlated with a poor prognosis of LAD patients. a, Relative expression of Forkhead box (Fox) genes in parental (SPC-A1 and H1299) and docetaxel-resistant LAD cells (SPC-A1/DTX and H1299/DTX) was determined by quantitative real-time PCR (qRT-PCR). GAPDH was used as an internal control. ⁎⁎P < 0.01. Data are representative of at least three independent experiments (means±standard deviation). b, Western blotting was used to detect protein expression of FOX-A1 in parental and docetaxel-resistant LAD cells. GAPDH was used as an internal control. ⁎⁎P < 0.01. Data are representative of at least three independent experiments (means±standard deviation). c, FOX-A1 gene expression was determined by qRT-PCR in parental LAD cells treated with different concentrations of DTX for 48 h. U6 was used as an internal control. ⁎P < 0.05, ⁎⁎P < 0.01. Data are representative of at least three independent experiments (means±standard deviation). d, qRT-PCR detection of FOX-A1 gene expression in parental LAD cells treated with DTX at different time points. U6 was used as an internal control. ⁎P < 0.05, ⁎⁎P < 0.01. Data are representative of at least three independent experiments (means±standard deviation). e, FOX-A1 expression detected by qRT-PCR in insensitive LAD tissues (CR + PR; n = 24) and sensitive LAD tissues (SD + PD; n = 28). GAPDH was used as an internal control. The cut-off value (0.959) of FOX-A1 mRNA expression in tumor tissues was determined by a receiver operating characteristic (ROC) curve.⁎⁎P < 0.01. f, FOX-A1 expression levels in normal lung tissues (n = 18) and LAD tissues (n = 52). FOX-A1 expression was detected by qRT-PCR. GAPDH was used as an internal control. ⁎⁎P < 0.01. g, Kaplan-Meier analysis of the correlation between FOX-A1 expression and progression-free survival (PFS) of LAD patients. ⁎⁎P < 0.01. h, Kaplan-Meier analysis of the association between FOX-A1 expression and overall survival (OS) of LAD patients.⁎⁎P < 0.01.