Figure 5.

BAG3 inhibits cisplatin-induced apoptosis in epithelial ovarian cancer cells. SKOV3 and SKOV3/DDP cells were transfected with control RNA or shRNA targeting BAG3, followed by treatment with 20 µM cisplatin for 48 h. (A) Apoptosis was analyzed by flow cytometry following Annexin V/propidium iodide staining. The percentages of Annexin V-positive cells are presented in the bar chart. The data are presented as the mean ± SD of three independent experiments. ***P<0.001. (B) Apoptosis was also determined using a TUNEL assay. Scale bar, 400 µm. The percentages of apoptotic cell are presented in the bar chart. **P<0.01. The data are presented as the mean ± SD of three independent experiments. BAG3, Bcl-2-associated athanogene 3; shRNA, short hairpin RNA; SD, standard deviation.