Figure 2.

Anti-oxidants enhance in vitro infection with L. braziliensis. Macrophages were infected with L. braziliensis for 24 h, and then exposed to different concentrations of NAC (A,B) and Apocycin (C,D) for 48 h. Cells were stained with H&E and assessed for (A,C) the percentage of infected macrophages and (B,D) the number of amastigotes per 100 macrophages by optical microscopy. Infected macrophages treated with Amphotericin B (AMB) were used as positive controls. Data are shown as mean ± SEM. ^*p < 0.05, ^**p < 0.01, all comparisons were against negative control (medium).