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. 2019 Jun 28;11:5799–5811. doi: 10.2147/CMAR.S193805

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© 2019 Ma et al.

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The mRNA expression profile of PRDX2 knockdown in HCT116 cells and DNM3 is a downstream protein of PRDX2. (A–C) PRDX2 siRNA was conducted and introduced into HCT116 cells. The mRNA expression profile was determined by RNA-Sequencing. The volcano plot (A) and heatmap (B) of different RNA expression profile, and the number of differently expressed genes (C). (D) The protein expression of PRDX2 and DNM3 was detected by Western blot in HT29, SW480, HCT116 and SW1116 cells. (E) The effects of PRDX2 knockdown or PRDX2 overexpression on DNM3 expression were tested by Western blot. The intensities of protein bands were quantified by Image J software. GAPDH was used as an internal control. *P<0.05. All experiments were performed in triplicate.

Abbreviations: DNM, Dynamin; DEGs, differently expressed genes; PRDX2-KD, cells transfected with siRNA-PRDX2 (PRDX2 knockdown); PRDX2-OV, cells transfected with pcDNA3.1-PRDX2 (PRDX2 overexpression); NC, negative control.