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. 2019 Jun 28;11:5799–5811. doi: 10.2147/CMAR.S193805

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© 2019 Ma et al.

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Dynamin 3 (DNM3) overexpression decreases cell viability in colon cancer cells. (A) DNM3 cDNA was cloned into pcDNA3.1 plasmid and introduced into HCT116 and HT29 cells, and the empty pcDNA3.1 plasmid was used as negative control (NC). The overexpression of DNM3 was validated by Western blot. The intensities of protein bands were quantified by Image J software. GAPDH was used as an internal control. (B) The effects of DNM3 overexpression on cell proliferation of HCT116 and HT29 cells were investigated by CKK-8 assay. (C) The cologenic ability was tested in DNM3 overexpressing HCT116 and HT29 cells. *P<0.05. All experiments were performed in triplicate.